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. 2019 May 9;14:3403–3411. doi: 10.2147/IJN.S192189

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© 2019 Liu et al.

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Cell vitality tests after GNS loading. MDSCs, DCs, and macrophages were incubated with or without TAT-GNS for 2 h or 16 h, respectively. Cells were then washed, stained for vitality markers, and detected by flow cytometry. (A, C, E) Representative plots for vitality staining of MDSC, DC, macrophages, respectively, after 2 h culture. (B, D, F) Quantification of AnnexinV- 7AAD- live cells for MDSC, DC, macrophages, respectively, after 2 h or 16 h culture. n=5. * p<0.05; ** p<0.01; **** p<0.0001.

Abbreviations: GNS, gold nanostars; MDSC, myeloid-derived suppressor cells; TAT, transactivator of transcription; DC, dendritic cells; ns, no significance.

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