Figure 4.

PBRM1 Expression Is Cytoprotective under High-Stress Conditions

PBRM1 induces apoptotic pathways and increases cellular viability under high-cellular-stress conditions.

(A) Depiction of the multifaceted role ROS regulators play in cancer.

(B) NMuMG cells were cultured in normal cell media or 250 μM H₂O₂ for 16 h, and the number of live cells were counted using trypan blue.

(C) Annexin V staining was measured in NMuMG cells treated with 200 μM H₂O₂ for the indicated times. The percentage of annexin V-positive cells was calculated for two independent experiments.

(D) Whole-cell lysates were prepared from NMuMG cells treated with 200 μM H₂O₂ for the indicated times and probed for the indicated proteins using immunoblot analysis.

(E) LDH release was measured from 10 μL media using LDH Cytotoxicity Assay Kit II (Abcam).

*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (paired Student's t test). ns, not significant. Error bars represent SD. Also see Figure S3.