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. 2019 Apr 24;20:9–21. doi: 10.1016/j.jare.2019.04.006

Fig. 3.

Fig. 3

LW6 in combination with gemcitabine (Gem) inhibits proliferation and induces cell death. The amount of BrdU incorporation was analyzed after treating 6606PDA (A) and MIA PaCa-2 (B) cells with the indicated concentrations of LW6 or 0.05 µM gemcitabine for 30 h. The combinational therapy, 80 µM LW6 plus 0.05 µM gemcitabine, significantly inhibited the proliferation of 6606PDA cells compared to the proliferation of the Sham-treated cells or that of the monotherapy-treated cells (A). Similar results were obtained after treating MIA PaCa-2 cells with 40 µM LW6 plus 0.05 µM gemcitabine (B). The percentage of dead cells was determined by a trypan blue assay after treating 6606PDA (C) and MIA PaCa-2 (D) cells with the indicated concentrations of LW6 or 0.05 µM gemcitabine for 48 h. LW6 plus gemcitabine treatment also significantly induced cell death compared to the levels of cell death induced in the Sham-treated or the monotherapy-treated cells (C and D). P ≤ 0.008 indicates a significant difference (SD).