Figure 2.

MaR1 inhibits carrageenan‐induced neutrophil and macrophage recruitment to the hind paw skin. Hind paw skin of LysM‐eGFP (neutrophil and macrophage marker, a) or Swiss (b–f) mice was dissected for determination of neutrophil (GR‐1 staining [b], MPO activity [d], and flow cytometry [CD11b⁺Ly6G⁺ cells, f]) and macrophage (CD68 staining [c], NAG activity [e], and flow cytometry [CD11b⁺F4/80⁺ cells, f]) recruitment 5 hr after carrageenan stimulus. LysM‐eGFP mouse, immunofluorescence, enzymatic activity, and flow cytometry data show that MaR1 reduced carrageenan‐induced recruitment of neutrophils and macrophages in the paw skin. Results are expressed as mean ± SEM, n = 6 mice per group per experiment, two independent experiments (*P < 0.05 vs. saline, ^# P < 0.05 vs. 0 mg·kg⁻¹ group; one‐way ANOVA followed by Tukey's post‐test)