Figure 5.

RANKL induces AR via increased TSLP levels. Mice were sensitized on Days 1, 5, and 14 by i.p. injections of 100 μg of OVA emulsified in 20 mg of aluminium hydroxide, after which they received local injections of TSLP siRNA and control siRNA, or PBS alone into the nasal cavity, and were challenged with intranasal RANKL for 10 days. (a) The number of the nose rubs that occurred 10 min after OVA intranasal provocation. (b) IgE levels in the serum were measured by elisa. (c) The levels of histamine in serum were measured as described. (d–h) The levels of cytokine in the serum were measured by elisa. Data shown are the means ± SEM from n = 5 mice per group. ^# P < 0.05; significantly different from OVA‐unsensitized mice. *P < 0.05; significantly different from control siRNA RANKL‐sensitized mice