Figure 9.

Iodide influx, expressed as the initial fluorescence quenching rate, QR, in FRT cells expressing WT‐CFTR (a), G551D‐CFTR (b), and F508del‐CFTR (c). Magenta bars indicate the control experiments with the vehicle DMSO (and no anionophore); the red bars and the blue bars are experiments with 2 μM prodigiosines and tambjamines respectively. As shown in each panel, light colours indicate cells incubated with only the anionophore (or the vehicle), and the darker coloured bars correspond to cells treated with the anionophore plus 20 μM forskolin. WT‐CFTR and G551D‐CFTR cells treated with the anionophore, forskolin, and 10 μM ivacaftor are indicated by the striped bars in (a) and (b) respectively. Cells with the mutant F508del‐CFTR pretreated with 5 μM lumacaftor overnight and incubated with the anionophore and forskolin are the striped bars in (c). Data are the mean ± SEM of 5‐12 separate measurements. † P < 0.05, significantly different from control (vehicle DMSO); *P < 0.05, significant effect of forskolin; ‡ P < 0.05, significant effect of forskolin with ivacaftor or lumacaftor respectively; Student's t test