Figure 2.

Aβ induction of PIAS1 phosphorylation at Ser⁵⁰³ is mediated through MAPK/ERK activation. (a) DMSO (15%) or U0126 (2 μg) was injected to rat hippocampal CA1 area, and the expression of pERK1/2, ERK1/2, pSer⁵⁰³ PIAS1, and PIAS1 was determined by Western blot 1 hr later. (b) The quantified results of these measures (n = 6 different animals for each group). ** P < 0.05 and ^# P < 0.05 for U0126 versus DMSO group. (c) DMSO (15%) or U0126 (1 μg) together with NH₄OH (1%) or Aβ (14 μg) was injected to rat hippocampal CA1 area 30 min apart, and the expression of pERK1/2, ERK1/2, pSer⁵⁰³ PIAS1, and PIAS1 was determined by Western blot 30 min after NH₄OH or Aβ injection. (d) The quantified results of these measures (n = 6 different animals for each group). * P < 0.05, ** P < 0.05, and ^# P < 0.05 comparing Aβ + DMSO versus NH₄OH + DMSO group and * P < 0.05, ** P < 0.05, and ^# P < 0.05 comparing Aβ + U0126 versus Aβ + DMSO group. Data are expressed as mean ± SEM