Figure 4.
Effect of hypoxia (1% O2, 24 hr) on PP2Ac activity (a, b), PP2Ac abundance (c, d), and PPP2CA mRNA expression (e, f) in HASMC and AC16 ventricular cardiomyocytes. Protein phosphatase activity is presented as a percentage of the phosphatase activity under normoxia. PP2Ac abundance and mRNA expression are normalized (β‐actin and geometric mean of GPI and GAPDH as appropriate) and expressed relative to values under normoxic conditions. Data are presented as the mean ± SEM (n = 5) and were analysed using an unpaired Student t‐test. *P < 0.05, significantly different as indicated. PP2Ac: catalytic subunit of protein phosphatase 2A; PPP2CA, gene coding PP2Ac