Fig. 7.

Leaf development and auxin responses in bps1 mutants. (A) Seven-day-old seedlings were either mock treated or supplied with 10 μM 2,4-D for 6 hours, and GUS stained for 12 hours, either with intact seedlings (top row) or with seedlings that had undergone root excision (bottom row). Auxin-induced DR5::GUS expression was observed only in bps1 leaf primordia following root excision. (B) GUS-stained leaf primordia of 5-day-old wild type and bps1 carrying pIAA18::IAA18-GUS. (C) PIN1-GFP patterns in leaf primordia of 3-day-old wild type and bps1 mutants. (D) Growth on NPA-supplemented media. NPA-treated bps1 leaf shows partial vein formation at the periphery of the leaf. Scale bars: 1 mm in the top row of D; 200 μm in A and the bottom row of D; 20 μm in C. in B 100 μm.