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. 2012 Jun 5;139(13):2351–2359. doi: 10.1242/dev.078485

Fig. 6.

Fig. 6.

An Ets binding site in a minimal FoxC enhancer is required to repress expression in rows III and IV of the neural plate. (A) Partial sequence of a minimal FoxC enhancer showing two putative Ets binding sites (blue boxes). (B) Ciona embryos were electroporated with plasmids DMRT>GFP to label the anterior neural plate and a FoxC minimal enhancer fragment (FoxC –375/–50 bp) cloned upstream of a heterologous promoter (from the FOG gene) driving lacZ. lacZ expression was detected by immunostaining with β-gal antibody (red). (C) Expression pattern of the minimal FoxC enhancer fragment from which the Ets site at –295 had been deleted. (D) Quantification of the results shown in B,C. Error bars indicate s.d. wt, wild type.