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. 2012 Apr 3;139(9):1620–1629. doi: 10.1242/dev.073056

Fig. 2.

Fig. 2.

Shh expression is downregulated in Hoxb6Cre; Isl1 cKO hindlimb buds. (A-D) Shh expression in the control hindlimb bud at the stages indicated. Shh expression initiates in a small posterior mesenchymal domain (A) and expands distally (B,C) and is downregulated in advanced stages (D). Black arrowheads indicate normal expression. (E-H′) In mutant hindlimb buds, no Shh expression was detected at E10.5 (E) and E10.75 (F). At E11.5 (G,G′) and E11.75 (H,H′), very low levels of Shh transcripts were detected. G′ and H′ are magnified views of the boxed areas in G and H. Red arrowheads and asterisks indicate reduced and no expression, respectively. (I) qRT-PCR analysis of Shh transcripts in hindlimbs at E11.5. The relative Shh levels in mutant hindlimbs (n=4, 0.6%) in comparison with control littermate hindlimb buds (n=4) is shown as average ±s.d.