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. 2012 Apr 3;139(9):1620–1629. doi: 10.1242/dev.073056

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Fig. 5. — Altered pelvis marker gene expression in Hoxb6Cre; Isl1 cKO hindlimb buds. (A-J) Analysis of Twist1 (A,F), Prrx1 (B,G), Pax1 (C,H), Pbx1 (D,I) and Pbx2 (E,J) expression in control (A-E) and mutant (F-J) hindlimb buds at E10.25-11.5. Twist1 expression is detected both in control (A) and mutant (F) hindlimb buds (arrow), but its posterior-proximal expression is downregulated in mutant hindlimb buds (asterisk, F). Prrx1 expression in the proximal region in control hindlimb buds (B, arrow) is lacking from mutant hindlimb buds (G, asterisk). Pax1 is normally expressed in the proximal-anterior region (C, arrow) and is lost from mutant hindlimb buds (H, asterisk). Pbx1 (D,I) and Pbx2 (E,J) is expressed in both control and mutant hindlimb buds (arrows).