Skip to main content
. 2012 Apr 3;139(9):1620–1629. doi: 10.1242/dev.073056

Fig. 6.

Fig. 6.

Hindlimb bud-specific gene expression is maintained in Hoxb6Cre; Isl1 cKO embryos. (A-L) Expression of Pitx1 (A,D,G,J), Hoxc10 (B,E,H,K) and Tbx4 (C,F,I,L) in control (A-F) and Hoxb6Cre; Isl1 cKO (G-L) embryos at E10.5. Lateral views (A-C,G-I) and dorsal views (D-F,J-L) are shown. Pitx1 was detected both in control (A,D) and Isl1 cKO (G,J) hindlimb buds. Hoxc10 was detected in control (B,E) and Isl1 cKO (H,K) hindlimb buds. Tbx4 was detected in control (C,F) and Isl1 cKO hindlimb buds (I,L). Arrows point to the expression in the hindlimb bud. f, forelimb buds; h, hindlimb buds. (M,N) qRT-PCR analysis of Pitx1 (M) and Tbx4 (N) transcripts in hindlimbs at E10.5. The relative transcript levels in two groups of mutants (n=6 in group1, n=4 in group2) in comparison with controls (n=6) are shown as average ±s.d. The same samples as for the analysis shown in Fig. 3 are used.