Quantification of heterogeneity and treatment response in PyVmT tumors and organoids with histology. (A) Automatic cell segmentation and quantification of Ki67 expression in 20x images of PyVmT tumor and organoids. Scale bar = 200 μm. N > 4 FOVs per tumor, 5 tumors per group. N > 39 organoids per group. (B) CC3 expression in tumors and organoids. N = 5 FOVs per tumor, 5 tumors per group. N > 44 organoids per group. (C) pHH3 expression in tumors and organoids. N = 5 FOVs per tumor, 5 tumors per group. N > 60 organoids per group. (D) CD45 expression in tumors and organoids. N > 4 FOVs per tumor, 5 tumors per group. N > 45 organoids per group. (E) αSMA expression in tumors and organoids. N > 4 FOVs per tumor, N > 5 tumors per group. N > 20 organoids per group. Error bars indicate mean ± SD. * P < .05 vs. control. All tumors and organoids were fixed after 48 hours and 72 hours of treatment, respectively.