Table 2.
Sequences for primers used in quantitative polymerase chain reaction (qPCR)
| Transcript | Forward | Reverse | Amplicon |
|---|---|---|---|
| β‐actin | ACCACACCTTCTACAATGAG | TAGCACAGCCTGGATAGC | 161 bp |
| GAPDH | ACCCACTCCTCCACCTTTGAC | TCCACCACCCTGTTGCTGTAG | 110 bp |
| CD3ε | CCGCCATCTTAGTAAAGTAACAG | AATACCACCCATTTCTTCATTACC | 131 bp |
| CD19 | GCTGGAAAGTATTATTGTC | TTGAAGATGAAGAATGCC | 177 bp |
| MS4A1 | GGGGCTGTCCAGATTATGAA | CCAGGAGTGATCCGGAAATA | 148 bp |
| IgG1 | CATCTCCAAAGCCAAAGG | ATGTCGCTGGGATAGAAG | 126 bp |
| IgG2‐4 | CATCTCCAAAGCCAAAGG | ATGTCGCTGGGGTAGAAG | 126 bp |
| IgM | CAGGGCACAGACGAACAC | CGGCAATCACTGGAAGAGG | 85 bp |
| CXCL13 | TCAGCAGCCTCTCTCCAG | GACTTGTTCTTCTTCCAGACTATG | 178 bp |
| CXCR5 | CGGCACAGCCATGAACTAC | CAATCTGTCCAGTTCCCAGAA | 82 bp |
| BAFF | CGTTCAGGGTCCAGAAGAAA | AAAGCTGAGAAGCCATGGAA | 115 bp |
GAPDH = glyceraldehyde‐3‐phosphate dehydrogenase; Ig = immunoglobulin; bp = base pairs; CCCL = C‐X‐C motif chemokine ligand; CXCR = C motif chemokine receptor; BAFF = B cell activating factor; bp: base pairs.