Table 1.
Genes | Sense primer (5′ → 3′) | Antisense primer (5′ → 3′) |
---|---|---|
r/hmiR‐34a | ACACTCCAGCTGGGTGGCAGTGTCTTAGCT | CTCAACTGGTCTCGTGGAGTCGGCAATTCAGTTGAGACAACCAG |
URP | TGGTGTCGTGGAGTCG | |
U6 | CTCGCTTCGGCAGCACA | AACGCTTCACGAATTTGCGT |
rp53 | GGGAATGGGTTGGTAGTT | AGAGTGGAGGAAATGGGT |
rGADPH | CCCCCAATGTATCCGTTGTG | TAGCCCAGGATGCCCTTTAGT |
hp53 | GTTCCGAGAGCTGAATGAGG | TCTGAGTCAGGCCCTTCTGT |
hGADPH | TGTGGGCATCAATGGATTTGG | ACACCATGTATTCCGGGTCAAT |
r/hmiR‐34a inhibitor | ACAACCAGCUAAGACACUGCCA | |
Scrambled control inhibitor | CAGUACUUUUCUCUAGUACAA | |
rP53 siRNA | GCUCCGACUAUACCACUAUTT | AUAGUGGUAUAGUCGGAGCTT |
hP53 siRNA | CUACUUCCUGAAAACAACGdTdT | CGUUGUUUUCAGGAAGUAGdTdT |
Scrambled control siRNA | UUCUCCGAACGUGUCACGUTT | ACGUGACACGUUCGGAGAATT |
r/hmiR‐34a mimics | UGGCAGUGUCUUAGCUGGUUGU | AACCAGCUAAGACACUGCCAUU |