Fig. 4.

Oocyte accumulation, similar to IIS downregulation, blocks tumorous germ cell proliferation. (A-I) Representative DAPI-stained (cyan) A3 to A4 animals of the indicated genotype, either grown under restrictive conditions (RC) from the L1 stage (top three panels), or grown under permissive conditions until the mid-L4 stage, then shifted to RC thereafter (bottom six panels) are shown. Alleles: conditional glp-1(ar202)gf, fog-1(q785), conditional daf-2(e1370), daf-18(nr2037), goa-1(n1134). Anterior is to the left and dorsal, to the top. A colour-coded quantification of the phenotype is shown on the right, scored at A4 (animals were scored as highly tumorous if more than one-third of the animal was translucid, slightly tumorous if between one-fifth and one-third was translucid, and non-tumorous if less than one-fifth of the animal was translucid; the relative lengths of the colour-coded bars represent percentages). Although glp-1(gf) animals are clearly tumorous already by A1-2 when grown under restrictive conditions (Pepper et al., 2003), animals are shown here at A3-4 to show that oocyte accumulation suppresses ectopic GSC proliferation for a prolonged duration. n= 28-52. Scale bar: 100 μm.