Fig. 6.

Independent regulation of GSC proliferation, and thereby of IIS outputs, in each gonad arm. (A-E) An oil diaphragm was micro-injected into the anterior or posterior spermatheca and/or uterus of fog-1(q785); glp-1(ar202)gf young adult hermaphrodites that had been grown under permissive conditions. Animals were then shifted to restrictive conditions and allowed to mate with males for 3 h (Fig. S1D). The germline phenotype was scored 2-4 days after injection in animals that successfully mated (based on progeny production). Oil was also micro-injected in the anterior or posterior spermatheca and/or uterus of gld-2(q497) gld-1(q485) young adults (these were not mated), which are non-conditional tumorous (Hansen and Schedl, 2013) and do not accumulate oocytes, to verify that the effect of the oil diaphragm was dependent on the presence of oocytes. A representative DAPI-stained animal is shown for each condition. Intact proximal-most oocytes are labelled with negative values from the proximal end in close-ups. Activated, but not fertilized, endomitotic oocytes are underlined by a dotted line. (A-C,D,E) Scale bar: 100 μm. (F) Summary of contraception experiments. Tum, tumorous. (G) A functional GFP::DAF-16 protein (Murphy et al., 2007) is predominantly cytoplasmic in well-fed A1 controls (top, 100%), as well as in A1 unmated fog-2(oz40) (middle, 96%) hermaphrodites, which have quiescent GSCs. Systemic IIS inactivation is not impaired in fog-2 mutants, as a 3 h-starvation period caused a rapid systemic nuclear enrichment of GFP::DAF-16 (bottom, 92%) in A1 fog-2 mutants. Similar results were observed in fog-1(q785) animals (data not shown). A representative animal is shown for each condition, cropped posterior to the pharynx, anterior to the left and dorsal up. Arrowheads point at some of the cells with obvious nuclear GFP::DAF-16 enrichment. n=24-41. Scale bar: 50 μm.