Fig. 8.

Use of the 4D atlas to stage Hematoxylin and Eosin histological sections. (A-D) By visual comparison, a comparable section (A) can be found in the 4D atlas for an approximately mid sagittal slice of nominal age E14 (B) by scanning over slice position, slice orientation and embryo age. Details from the images in A and B are shown in C and D, respectively. Identification of the corresponding 4D atlas slice enables identification of the age as E14.05. (E,F) Another example with a younger embryo of nominal age ∼E11 that corresponds to E11.50 in the 4D atlas. Scale bar: 1 mm.