xnd-1 is essential for germ cell proliferation. Photomicrograph of age-matched adult animals stained with DAPI to show the germ lines (dotted lines, distal to the left). (A) Zygotic loss of xnd-1 led to reduced germline size. Compare wild type (top) with the smaller xnd-1 M+Z− (bottom). (B) Comparison of germline size in xnd-1 M−Z+ (top) versus xnd-1 M−Z− (bottom) revealed XND-1 zygotic rescue of germ cell proliferation defects. Anterior is to the left. Scale bars: 20 µm. mit, mitotic zone; TZ, transition zone; P, pachytene; Dp, diplotene.