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. 2017 Feb 28;144(5):778–783. doi: 10.1242/dev.139493

Fig. 2.

Fig. 2.

TRBP increases Notch-dependent transcription. (A-F) mRNA expression levels of each indicated Notch target gene as measured by qRT-PCR, 2 days after E14.5 primary neural progenitor cells were transduced with retroviral vectors expressing (A-C) TRBP or (D-F) shTRBP. (G,H) Luciferase assay was used to detect the activation of a Notch reporter consisting of firefly luciferase fused to a CBF1-responsive element. (G) NIH3T3 or (H) E14.5 primary neural progenitor cells were transfected with a Notch reporter and the indicated expression vectors. Cells were harvested at 2 days post-transfection for luciferase assays. (I,L) Anti-GFP immunolabeling of E15.5 brain sections electroporated in utero with the indicated expression vectors at E13.5. (J,M) Quantification of I and L, respectively. (K) Co-IP assays using HEK 293T cells showing that a TRBP mutant (TRBPΔC) is deficient in binding to Dicer. Scale bars: 50 µm. Error bars represent s.d. *P<0.05, **P<0.01, ***P<0.001, Student's t-test.