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. 2015 Jan 6;142(2):291–302. doi: 10.1242/dev.115147

Fig. 3.

Fig. 3.

Loss of rfp-1 enhances overproliferation of glp-1(ar202gf). Gonads immunostained with anti-REC-8 antibodies (green) for proliferative cells, anti-HIM-3 antibodies (red) for meiotic cells, and with DAPI (blue) for germ cell nuclei. Gonads are outlined and an asterisk marks the distal end of each gonad. (A) N2 (wild-type) germ line. (B,C) Germ line of glp-1(ar202gf) single-mutant animals fed with either OP50 bacteria (B) or pL4440 RNAi vector only (C). These germ lines have an enlarged PZ, with no ectopic proliferative cells in the proximal germ line. (D) Germ line of glp-1(ar202gf) single mutant fed with rfp-1(RNAi). Large patches of proliferative cells are found throughout the germ line, including in the proximal germ line. (E) rfp-1(ok572) single mutant has a smaller PZ, with an average of 165 germ cells compared with 207 in the wild-type (N2) PZ (Table 2); however, germline polarity is similar to wild type. (F) rfp-1(ok572) glp-1(ar202gf) double mutant has proliferative cells throughout, interspersed with small patches of meiotic germ cells. Scale bar: 50 μm.