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. 2014 Sep 23;141(19):3709–3720. doi: 10.1242/dev.112185

Fig. 2.

Fig. 2.

The intracellular domain of Sema6B is essential for post-crossing commissural axon guidance. (A) Schematics of miRNA and rescue constructs used in B-H. (B-G″) Open-book analysis of DiI injection sites in embryos co-electroporated with: (B) miLuc and pMES; (C) miS6B and pMES; (D) miS6B and pSema6BΔmiR; (E) miS6B and pSema6BΔCTΔmiR; (F) pSema6BΔmiR alone; or (G) pSema6BΔCTΔmiR alone. Arrows (B,D,F) indicate normal crossing and turning of commissural axons. Post-crossing axons that failed to turn correctly at the contralateral floorplate border are indicated by arrowheads (C,E,G). (B′-G′) Merge of DiI-labeled axons (red) and EGFP (green) used to visualize the expression of pMES constructs. (B″-G″) Enhanced blue fluorescent protein-2 (EBFP2) visualizes the expression of miRNA constructs. The floorplate is indicated by dashed lines. Scale bars: 100 µm. (H) Quantification of DiI injection sites with normal axonal pathfinding. **P<0.01, ***P<0.001; n.s., not significant; error bars indicate s.e.m.