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. 2018 Oct 5;145(19):dev170019. doi: 10.1242/dev.170019

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© 2018. Published by The Company of Biologists Ltd

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Fig. 4. — Apoptosis in Spca1^1D mutants. (A,B) MicroCT scans of E8.75 wild-type and Spca1^1D embryos (dorsal views). White arrowhead indicates midbrain area, unfilled arrowhead hindbrain area. (C-H) Immunodetection of activated caspase 3 (αCasp3) in wild-type and Spca1^1D embryos at E8.75 (C,D), E9.5 (E,F) and E10.5 (G,H). (I) Plot represents average apoptotic index (activated caspase 3 foci/total number of nuclei) in wild-type and Spca1^1D embryos at E8.75, E9.5 and E10.5 (n=10 sections from three biological replicates for each stage and genotype). HM, head mesenchyme; NT, neural tube. Error bars indicate s.e.m. Scale bars: 300 µm (A,B); 100 µm (C-H).