Skip to main content
. 2018 Mar 15;145(6):dev156612. doi: 10.1242/dev.156612

Fig. 2.

Fig. 2.

DNMT3L expression and de novo re-methylation of the germ cell genome is delayed in teratoma-susceptible strains. (A) Oct4ΔPE::GFP transgenic FVB, 129 and M19 germ cells from E13.5-E15.5 were FACS-enriched and analyzed by qPCR (n=4-8) for Dnmt3l expression. Female germ cell expression data from all strains were pooled. Gene expression at all embryonic time-points was plotted relative to expression in E13.5 FVB germ cells. Data are mean±s.e.m. *P<0.05, †P<0.01 and ‡P<0.001. (B-E) Oct4ΔPE::GFP transgenic FVB and M19 embryonic testes were sectioned and immunostained for (C) DNMT3L or (E) 5-methyl cytosine (5-meC). Oct4ΔPE::GFP-positive germ cells, which are positive and negative for DNMT3L or 5-meC, were counted. Data are plotted as the average percentage of germ cells positive for (B) DNMT3L or (D) 5-meC ±s.e.m. (n=3-7). †P<0.01 and #P<0.0001. (C,E) Confocal microscopy images of representative E15.5 Oct4ΔPE::GFP transgenic FVB and M19 gonads immunostained for (C) DNMT3L or (E) 5-meC. Boxed areas are shown at higher magnification on the right. Scale bars: 50 μm.