Fig. 6.

The glial subtype markers Apontic and Gli-lacZ are altered after focal adhesion loss. An antibody to the transcription factor Apontic (Apt, green) and Gli-lacZ (blue) labeled PG and WG nuclei, respectively. Glial membranes were labeled using repo>CD8::RFP (red). Orthogonal sections were made from the marked positions. (A) In controls (Gli-lacZ; repo>CD8::RFP), Apt immunolabeling was detected in all peripheral PG nuclei (arrowhead) and β-galactosidase immunolabeling was restricted to WG nuclei (arrow). (B-D) In Gli-lacZ; repo>βPS-RNAi most PG and WG nuclei were differentially labeled by either Apt or Gli-lacZ. However, some glia expressed both markers (arrowheads) or expressed neither (double arrows). Note that some Gli-lacZ-positive WG nuclei (arrows) were observed beyond axonal stalling regions in B and C.