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. 2019 May 7;13:186. doi: 10.3389/fncel.2019.00186

Figure 7.

Figure 7

Spontaneous and ACh evoked [Ca2+]i transients in utricular maculae from mature mice. (A) Spontaneous G5 ΔF/F0 recorded at 100ms-frame−1 in the control condition. Waterfall plot shows transients within 3μm diameter ROIs. Solid curve above the waterfall shows the average over all ROIs. A1-2: examples of ROIs within cells of calyceal morphology near the base of HCs, and A3: example with bouton morphology. (B) Same ROIs modulating in response to 1s puff application of 100μM ACh. ACh increased the rate of transient events and evoked a slow rise in [Ca2+]i (large gray arrows). B1-3: same ROIs as (A), but with transients evoked by ACh. B3: ACh extended the spatial extent of the transient relative to spontaneous events. Solid curve above the waterfall shows the average over all ROIs. (C,D) Population statistics demonstrating that ACh evoked a significant increase in the rate of fast transients as well as a slow increase in [Ca2+]i. No change was detectable in kinetics, duration, or magnitude of fast transients in the ACh condition relative to controls. *p = 0.05.