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. 2019 May 7;13:186. doi: 10.3389/fncel.2019.00186

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Copyright © 2019 Holman, Poppi, Frerck and Rabbitt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Summary of spontaneous and ACh evoked [Ca²⁺]_i transients with age. (A) Cartoon depicting a supporting cell (SC), extrastriolar type I tdT positive hair cell (HC), calyx ending (C), immature proto-calyx ending (PC), and small ACh activated domains (R) near the base of HCs. Focal planes are denoted i-iii. (B) Intense spontaneous ΔF/F₀ transients in immature HCs at P1. (C) Intense spontaneous ΔF/F₀ transients in SCs and additional transients in HCs and PCs at P3-5. ACh synchronized of transients at P5. (D) Sparse spontaneous transients in SCs, intense ACh evoked transients in some HCs, and additional transients in Cs and Rs in P8 and older mice. (E) Spontaneous transients in SCs and intense ACh-evoked transients C endings.