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. 2016 Feb 17;219(4):544–552. doi: 10.1242/jeb.131862

Fig. 2.

Fig. 2.

The number of mitotic blastomeres decreases as embryos progress towards DII. Embryos were fixed and stained with anti-histone H3 serine 10 phosphate (H3S10P) to detect mitotic nuclei and Hoechst 33342 to stain DNA. (A) The anterior tail region is shown for all images: 18 dpf represents a preDII embryo, 24 dpf represents an embryo in early DII, and 32 dpf is a DII embryo. Scale bar, 50 µm. Representative embryos are shown (at least 18 embryos were analyzed; ≥6 embryos per trial, N=3, for each embryo stage). (B) The number of positive H3S10P cells was quantified in the tail region of embryos at various ages of preDII and DII. The data represent at least 18 embryos (analysis of ≥6 embryos per trial, N=3, for each embryo stage). The values are means±s.e.m. Identical letters indicate embryo groups with no significant difference; different letters indicate P<0.05. The P-values were determined using one-way ANOVA followed by a Holm–Sidak multiple comparison test.