Fig. 5.

mRNA expression of genes by RT-PCR, β-Actin, BDNF, Shh, GLI1, GLI2, GLI3, NKX2.2, PAX6 and β-catenin in rat hippocampus, all the gels were run separately and grouped as Fig. 5A, M- Marker/DNA ladder of 1000 bp, N-Negative control. The qRT-PCR mRNA expression showed as (5B) BDNF mRNA expression, Shh (5C), GLI2 (5D), GLI3 (5E), NKX2.2 (5F), PAX6 (5G) and β-catenin (5H). Results are expressed as Mean ± SE (n = 3). Significant differences between CON vs NIC is expressed as •; CON vs CUMS is expressed as *; NIC vs CUMS is expressed as ⊕ and CUMS vs CUMS + NIC is expressed by #. p value <0.05 considered as significant.