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Gene expression analysis in the UCB HSPCs using cDNA subtractive hybridization. The procedure is demonstrated from separating mononuclear cells (MNCs) from the umbilical cord blood (UCB) and isolating hematopoietic stem/progenitor cells (HSPCs) to the final list of genes that were found to be different in PE. The HSPCs (CD34+ CD45+) were sorted during SAM analysis and were used in this experiment (PE, n = 5 and NO, n = 10).
