Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Apr 25;20(8):2044. doi: 10.3390/ijms20082044

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

β-catenin immunohistochemistry on osteocytes in the tibial trabecular bone. (A) Protein expression of β-catenin in the trabecular bone of rats was determined by IHC assay. Rats from the CKD + exercise group showed a higher β-catenin staining signal on osteocytes than in case of rats from the CKD group. The arrows indicate the positive cells. The arrowheads indicate the negative cells. (B) Representative images of tissue cytometry using HistoQuest software. β-catenin-immunoreactive (IR) positive and negative cells were counted and the signal intensity was quantified. (C) Images are representative and quantification data are presented as the mean ± SD from 5 rats in each group. The CKD group showed a significant difference compared to the CKD + exercise and sham groups (**** p < 0.0001). The CKD + exercise group showed no significant difference compared to the sham group. The analysis was performed using the One way-ANOVA test. (D) 40× and (E) 100× images of the negative control without primary antibody.