Table 1.
Phenotype characteristics of the S17 isolate on different media, and C sources with and without the addition of Cu and the indoleacetic acid (IAA) precursor—tryptophan (Trp). Data are means ± SD (n = 3). Abbreviations: MM9 = minimal medium; LB = Luria–Bertani broth; Trp−Cu = medium with Trp and without Cu; −Cu = medium without Cu; +Cu = medium with 50 µM Cu; Fe(III)CC = Fe(III) complexing compounds; CAS = chrome azurol S blue medium; psu = percentage siderophore unit; nd = not detected.
| Parameter/ Metabolite |
Incubation Time | Medium/Treatment | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| MM9 Glucose | MM9 Sucrose | LB | ||||||||
| +Trp −Cu |
−Trp −Cu |
−Trp +Cu |
+Trp −Cu |
−Trp −Cu |
−Trp +Cu |
+Trp −Cu |
−Trp −Cu |
−Trp +Cu |
||
| Growth (OD600) |
24 h | 0.037 ± 0.007 | 0.027 ± 0.006 | 0.006 ± 0.001 | 0.028 ± 0.001 | 0.012 ± 0.004 | 0.001 ± 0.001 | 1.221 ± 0.011 | 1.229 ± 0.001 | 1.282 ± 0.026 |
| 48 h | 0.095 ± 0.001 | 0.101 ± 0.002 | 0.054 ± 0.001 | 0.084 ± 0.007 | 0.079 ± 0.006 | 0.004 ± 0.003 | 1.274 ± 0.029 | 1.252 ± 0.023 | 1.299 ± 0.002 | |
| Proteins (µg·mL−1) |
24 h | 5.79 ± 1.46 | 1.94 ± 1.14 | 4.87 ± 1.56 | 3.10 ± 1.60 | 2.58 ± 1.65 | 6.69 ± 1.75 | 8.62 ± 2.87 | 3.65 ± 1.46 | 5.24 ± 1.26 |
| 48 h | 7.56 ± 1.48 | 3.29 ± 0.09 | 2.68 ± 0.31 | 2.41 ± 0.24 | 1.79 ± 0.04 | 2.12 ± 1.47 | 7.91 ± 0.21 | 12.31 ± 1.65 | 7.58 ± 1.29 | |
| pH | 24 h | 3.80 ± 0.05 | 3.90 ± 0.02 | 3.89 ± 0.03 | 3.74 ± 0.06 | 3.69 ± 0.09 | 3.82 ± 0.02 | 8.44 ± 0.15 | 8.54 ± 0.12 | 8.52 ± 0.10 |
| 48 h | 3.24 ± 0.01 | 3.21 ± 0.04 | 3.79 ± 0.01 | 3.70 ± 0.05 | 4.39 ± 0.03 | 3.69 ± 0.02 | 8.97 ± 0.14 | 8.92 ± 0.21 | 8.90 ± 0.13 | |
| IAA (µg·mL−1) |
24 h | 4.95 ± 0.02 | 0.00 ± 0.00 | 0.00 ± 0.00 | 6.50 ± 0.40 | 0.00 ± 0.00 | 0.12 ± 0.02 | 3.75 ± 0.19 | 1.53 ± 0.12 | 1.40 ± 0.07 |
| 48 h | 3.70 ± 0.03 | 0.43 ± 0.13 | 0.20 ± 0.02 | 2.60 ± 0.09 | 0.00 ± 0.00 | 0.00 ± 0.00 | 9.35 ± 0.28 | 3.70 ± 0.03 | 5.13 ± 0.02 | |
| Fe(III)CC FeCl3 test (µg·mL−1) |
24 h | 5.31 ± 0.11 | 5.05 ± 0.09 | 4.93 ± 0.01 | 6.41 ± 0.38 | 5.49 ± 0.05 | 4.93 ± 0.01 | 3.79 ± 0.20 | 4.03 ± 0.07 | 1.54 ± 0.12 |
| 48 h | 5.21 ± 0.01 | 6.23 ± 0.07 | 4.93 ± 0.06 | 5.07 ± 0.03 | 4.94 ± 0.18 | 4.76 ± 0.03 | 5.49 ± 0.50 | 6.90 ± 0.07 | 3.35 ± 0.19 | |
| Siderophores total CAS test (psu unit) |
24 h | 8.09 ± 0.08 | 3.47 ± 0.04 | 1.60 ± 0.08 | 15.01 ± 0.07 | 5.75 ± 0.01 | 0.49 ± 0.01 | 51.80 ± 0.02 | 51.01 ± 0.04 | 47.08 ± 0.02 |
| 48 h | 19.98 ± 0.02 | 15.92 ± 0.15 | 1.57 ± 0.08 | 20.80 ± 0.06 | 14.62 ± 0.03 | 0.89 ± 0.07 | 62.01 ± 0.02 | 71.10 ± 0.01 | 62.84 ± 0.03 | |
| Hydroxamate siderophores (µg·mL−1) |
24 h | 5.29 ± 0.84 | 2.46 ± 0.10 | 0.88 ± 0.07 | 1.81 ± 0.37 | 0.32 ± 0.06 | 0.21 ± 0.17 | 7.42 ± 0.64 | 0.35 ± 0.03 | 0.23 ± 0.05 |
| 48 h | 2.16 ± 0.24 | 4.29 ± 0.58 | 0.23 ± 0.03 | 2.37 ± 0.17 | 0.57 ± 0.03 | 1.50 ± 1.15 | 5.56 ± 0.32 | 1.64 ± 0.03 | 0.19 ± 0.01 | |
| Catechole siderophores (µg·mL−1) |
24 h | 1.21 ± 0.04 | 1.02 ± 0.01 | 1.09 ± 0.13 | 1.25 ± 0.19 | 1.05 ± 0.02 | 1.20 ± 0.17 | 1.15 ± 0.03 | 2.45 ± 0.12 | 1.19 ± 0.13 |
| 48 h | 1.05 ± 0.03 | 0.99 ± 0.10 | 0.98 ± 0.05 | 1.00 ± 0.04 | 0.92 ± 0.01 | 1.12 ± 0.03 | 0.93 ± 0.04 | 0.97 ± 0.17 | 1.19 ± 0.01 | |
| Phenolic compounds (µg·mL−1) |
24 h | nd | 25.04 ± 0.68 | 14.61 ± 0.20 | nd | 14.74 ± 0.76 | 16.32 ± 2.28 | nd | nd | nd |
| 48 h | nd | 20.18 ± 0.01 | 25.32 ± 1.71 | nd | 17.30 ± 1.37 | 30.80 ± 1.31 | nd | nd | nd | |