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. 2019 Apr 17;20(8):1909. doi: 10.3390/ijms20081909

Table 2.

Total flavonoids (FLAVO), total phenolic compounds (TPC), and antioxidant capacity (AC) determined using ABTS (2-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid) and DPPH (2,2-diphenyl-1-picrylhydrazyl) in the Phaseolus coccineus leaves and roots. Data are means ± SD (n = 3). Values in each column followed by the same letter are not significantly different (p < 0.05; Tukey’s test). Abbreviations: J0 = without methyl jasmonate (J); J1 = with 1 µM of J; J10 = with 10 µM of J; −Cu = without Cu; +Cu = with 50 µM Cu; NI − S17 = no inoculation with the S17 isolate; I + S17 = inoculation with the S17 isolate; FW = fresh weight.

Treatment FLAVO TPC AC
ABTS DPPH
mg·g−1·FW
Leaf Root Leaf Root Leaf Root Leaf Root
J0 −Cu NI − S17 0.22 ± 0.02 e,f 0.012 ± 0.001 d 0.72 ± 0.06 e,f 0.25 ± 0.02 e,f 0.33 ± 0.01 d 0.025 ± 0.002 f 0.074 ± 0.007 g,h 0.006 ± 0.001 f
J1 −Cu NI − S17 0.22 ± 0.02 e,f 0.016 ± 0.002 d 0.57 ± 0.05 f,g 0.26 ± 0.03 e,f 0.30 ± 0.03 d 0.033 ± 0.002 e,f 0.061 ± 0.006 g,h 0.003 ± 0.000 f
J10 −Cu NI − S17 0.29 ± 0.02 d 0.012 ± 0.001 d 0.91 ± 0.08 d,e 0.22 ± 0.02 e,f 0.61 ± 0.06 c 0.026 ± 0.001 f 0.203 ± 0.018 d,e 0.004 ± 0.000 f
J0 +Cu NI − S17 0.40 ± 0.02 b,c 0.055 ± 0.005 a 1.52 ± 0.09 b 0.75 ± 0.03 a 0.78 ± 0.03 a,b 0.133 ± 0.007 a 0.305 ± 0.030 a,b 0.093 ± 0.011 a
J1 +Cu NI − S17 0.24 ± 0.02 d,e 0.038 ± 0.003 c 1.16 ± 0.11 c 0.66 ± 0.03 b,c 0.56 ± 0.06 c 0.116 ± 0.005 b 0.114 ± 0.009 f,g 0.059 ± 0.006 c,d
J10 +Cu NI − S17 0.36 ± 0.03 c 0.054 ± 0.003 a 1.44 ± 0.14 b 0.67 ± 0.04 a,b,c 0.55 ± 0.05 c 0.120 ± 0.006 a,b 0.338 ± 0.033 a 0.077 ± 0.007 b
J0 −Cu I + S17 0.17 ± 0.02 f 0.012 ± 0.000 d 0.47 ± 0.05 g 0.20 ± 0.01 f 0.33 ± 0.02 d 0.032 ± 0.003 e,f 0.050 ± 0.005 h 0.004 ± 0.000 f
J1 −Cu I + S17 0.24 ± 0.02 d,e 0.015 ± 0.003 d 0.66 ± 0.06 f,g 0.29 ± 0.03 d,e 0.35 ± 0.04 d 0.041 ± 0.003 d,e 0.088 ± 0.008 g,h 0.008 ± 0.001 f
J10 −Cu I + S17 0.24 ± 0.02 d,e,f 0.016 ± 0.002 d 0.63 ± 0.05 f,g 0.35 ± 0.03 d 0.42 ± 0.02 d 0.051 ± 0.005 d 0.101 ± 0.011 f,g,h 0.003 ± 0.000 f
J0 +Cu I + S17 0.56 ± 0.04 a 0.055 ± 0.003 a 1.77 ± 0.11 a 0.66 ± 0.03 b,c 0.81 ± 0.06 a 0.103 ± 0.005 c 0.273 ± 0.029 b,c 0.030 ± 0.001 e
J1 +Cu I + S17 0.44 ± 0.02 b 0.047 ± 0.002 b 1.51 ± 0.13 b 0.73 ± 0.06 a,b 0.67 ± 0.06 b,c 0.133 ± 0.005 a 0.222 ± 0.010 c,d 0.070 ± 0.003 b,c
J10 +Cu I + S17 0.36 ± 0.02 c 0.032 ± 0.002 c 0.96 ± 0.08 c,d 0.59 ± 0.06 c 0.63 ± 0.03 c 0.131 ± 0.005 a 0.153 ± 0.026 e,f 0.051 ± 0.005 d