Table 2.

Kinetic parameters of free and immobilized enzyme, amount of immobilized enzyme and immobilization yield.

Kinetic Parameters and Immobilization Data	Free Laccase	ZrO2–SiO2–Laccase	ZrO2–SiO2/Cu2+–Laccase
Km (mM)	0.049 ± 0.002	0.132 ± 0.009	0.098 ± 0.008
Vmax (U/mg)	0.041 ± 0.006	0.029 ± 0.007	0.037 ± 0.009
Amount of enzyme (mg/g)	-	86 ± 3.8	94 ± 3.2
Immobilization yield (%)	-	86 ± 3.9	94 ± 3.1