. 2019 Apr 22;20(8):1959. doi: 10.3390/ijms20081959

Table 2.

ROS and their mode of detection using fluorescent probes.

ROS	Fluorescent Probe	Examples
Hydrogen peroxide	H₂O₂-induced oxidation of arylboronate ester to phenols.	(Ref: 1 [98], 2 [99])
Superoxide	Nucleophilic substitution reaction of superoxide with the probes where the leaving group is phosphinate group [104], triflate group [105], 2,4-dinitrobenzenesulfonyl [106], nitro-ethers [107] and the related groups.	(Ref: 3 [128],4 [108])
Hydroxyl radical	Hydroxyl radical-mediated oxidation of leuco forms of fluorescent dyes and nitroxide moiety.	(Ref: 5 [112], 6 [129])
Singlet oxygen	The fluorescent probes for singlet oxygen have been developed based on the [2 + 4] cycloaddition where singlet oxygen acts as a strong dienophile [109,114,115].	(Ref: 7 [115])
Peroxynitrite	Peroxynitrite-mediated oxidation of chalcogenides (S, Se, and Te), boronic acids or boronates, hydrazides, cleavage of C–C double bonds and oxidative N-dearylation.	(Ref: 8 [122])
Nitric oxide	NO-mediated reaction of O-diaminophenyl group with NO to generate the triazole.	(Ref: 9 [130])
Hypochlorite	Hypochlorite mediated spiro-ring opening of the xanthene probes and oxidation of catechol to benzoquinone [122].	(Ref: 10 [117])