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. 2019 Apr 15;20(8):1863. doi: 10.3390/ijms20081863

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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RNA-Seq analyses and RT-qPCR validation of the ROS network genes in M85 and CRI12 under alkali-salt stress. (A) The number of differentially expressed genes (DEGs) of G. hirsutum races marie-galant (M85) and G. hirsutum cultivar (CRI12) under complex alkali-saline stress at 3, 12, and 48 h. Red block is up-regulated DEGs. Green block is down-regulated DEGs. (B) PCA (principal component analysis) plot of the first two components for genes of ROS network. The dot color scheme represents different cotton accession (TM1, red; M85, yellow; CRI12, blue.). (C) Correlation coefficients of TM-1, CRI12, and M85. (D) RT-qPCR analysis of selected ROS genes, 1, 2, and 3 refers to the libraries, yellow-upregulated, blue: down-regulated, and black: no expression.