ROS network mediated signal pathway to illuminate the mechanism of fiber development and regulation of abiotic stress in Gossypium. Red box indicates modulation of ROS level by the reactive oxygen gene network of plants. ROS network regulated fiber development and abiotic stress by control ROS level, but it is not clear about how to do it. RNA-seq data of TM-1, CRI12 and M85 proved that serine/threonine kinase (OXI), and motigen-activated protein kinase (MAPK) cascades might involve in the alkali-salt stress (one of abiotic stresses) response which ROS network mediate. Under fiber development-box, heatmap of some ROS network genes indicted genes expression levels at 10 and 20 DPA (A, G. arboreum; D, G. raimondii; At, G. hirsutum A subgenome; Dt, G. hirsutum D subgenome) by R package (Pheatmap, scale = “row”). Under abiotic stress-box, heatmap indicated value of genes expression levels fold change (log2 (FC)) under cold, hot, dehydration and salt stress by Pheatmap package.