Skip to main content
. 2019 Mar 29;294(19):7769–7786. doi: 10.1074/jbc.RA119.007386

Figure 4.

Figure 4.

EpEX suppresses miRNA, let-7, through EGFR–STAT3–Lin28 signaling. A, cells were treated with EpEX (3 μg/ml), and the expression of let-7 was detected by qPCR. B and C, MSCs were pretreated with or without AG1478, or WP1066, and then stimulated with EpEX. The expression of let-7 was detected by qPCR. D–F, MSCs expressing EGFR shRNA, STAT3 shRNA, or Lin28b shRNA were stimulated with EpEX. The expression of let-7 was detected by qPCR. G, MSCs were transfected with a let-7 inhibitor or a let-7 mimetic, and then stimulated with EpEX. Expression of pluripotency factors (Oct4, Sox2, c-Myc, and Lin28) was examined by qPCR. H, MSCs were transfected with a let-7 mimetic, and then stimulated with EpEX. Protein levels of pluripotency factors (Oct4, Sox2, c-Myc, and Lin28) were examined by Western blotting. Data represent the mean ± S.D. *, p < 0.05.