Figure 6.
Proteasome isoforms overall share a common substrate cleavage-site usage with specific differences. The correlation between the SCSs of the synthetic substrates gp100201–230 (pink circles), gp10035–53 (blue circles), MBP102–129 (green circles), and MOG172–202 (black circles) generated in in vitro digestion kinetics by human 20S s-, i-, or t-proteasomes is shown. The SCSs are compared in proteasomes pairwise. The computed Pearson's correlation coefficients and the tests for significance of these correlations are reported. Nonsignificant correlations are marked in bold and depict SCSs that differ between the pair of compared 20S proteasome types. The substrate cleavage-sites which the use of is more remarkably varied between 20S proteasome isoforms are labeled.