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Proteasome isoforms generate MBP epitopes with different kinetics. The degradation kinetics of the synthetic substrates MBP102–129 by 20S s-, i-, and t-proteasomes and the generation kinetics of the epitopes MBP111–119 and MBP107–115 are shown. The mean of two to four independent experiments (error bars are the S.D.) measured in duplicate is shown. Quantitative analyses are done by applying QME to the MS measurements.
