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. 2019 Mar 26;294(19):7864–7877. doi: 10.1074/jbc.RA118.006166

Figure 2.

Figure 2.

4-MUG is converted into 4-MU in vitro. A, fluorescence visualization in wells of a 96-well plate which was filled with 200 μl PBS and 10% FCS. In some wells 4-MU (middle) and 4-MUG (right) were added; control wells remained untreated (left). B, 4-MU and 4-MUG were separately added to DMEM and their fluorescent signal over time was measured as mean fluorescent intensity (MFI). Fluorescent values of 4-MUG were normalized to the 4-MU fluorescence. C, fluorescence of 4-MU and 4-MUG from B16F10 cells incubated for 24, 48, or 72 h with 4-MU and 4-MUG examined using flow cytometry. D, fluorescence of 4-MU and 4-MUG signal from 4-MU– and 4-MUG–treated B16F10 cells pre- and post-permeabilization was examined by flow cytometry.