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ppITAM binding accelerates LYN-mediated phosphorylation of kinase dead-SYK (SYK-KD). SYK-KD was treated with LYN, ATP, and with or without ppITAM for the indicated times. Reactions were subjected to Western blotting with phosphosite-specific antibodies as described under “Experimental procedures.” The region migrating at 65–80 kDa is shown.
