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. 2019 Mar 28;294(19):7850–7863. doi: 10.1074/jbc.RA119.008074

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© 2019 McGregor et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 3. — Release of soluble IL-6R by osteocytes in vitro. Shown are sclerostin (Sost; A and D) and IL-6 receptor (Il6ra; B and E) mRNA levels and sIL-6R protein levels (C and F) in cell lysates and conditioned media from differentiating Ocy454 cells (A–C) and Kusa4b10 cells (D–F). Data are the mean ± S.E. (error bars) from three independent preparations. *, p < 0.05, **, p < 0.01, ***, p < 0.001 versus undifferentiated cells by one-way ANOVA with Tukey post hoc test; C and F do not contain statistics because levels were undetectable at day 0.