Table 2.
Hemizygous PEPCK-sgp130-Fc mice have normal trabecular and cortical bone mass
Data from 12-week-old male and female PEPCK-sgp130-Fc mice (hemizygotes) are shown as mean ± S.E. No significant differences associated with the PEPCK-sgp130-Fc genotype were detected in any parameters, assessed by two-way ANOVA. Sex differences were observed in all parameters in both control and PEPCK-sgp130-Fc mice: *, p < 0.05; **, p < 0.01; ***, p < 0.001 versus female control; +, p < 0.001 versus sex-matched control.
| Female |
Male |
|||
|---|---|---|---|---|
| Control (n = 8) | PEPCK-sgp130-Fc (n = 8) | Control (n = 8) | PEPCK-sgp130-Fc (n = 7) | |
| Serum human gp130 (μg/ml) | Undetectable | 4.756 ± 1.34+ | Undetectable | 2.410 ± 0.37+ |
| Trabecular bone | ||||
| Trabecular bone volume (%) | 8.14 ± 0.61 | 7.01 ± 0.92 | 19.76 ± 1.44*** | 20.55 ± 1.80*** |
| Trabecular thickness (μm) | 45.1 ± 0.6 | 43.6 ± 0.9 | 53.4 ± 1.1*** | 54.5 ± 1.2*** |
| Trabecular number (/mm) | 1.80 ± 0.12 | 1.60 ± 0.18 | 3.68 ± 0.21*** | 3.74 ± 0.26*** |
| Trabecular separation (μm) | 248.6 ± 4.8 | 279.5 ± 16.6 | 167.9 ± 4.8*** | 164.4 ± 9.6*** |
| Bone size and shape | ||||
| Femoral length (mm) | 14.18 ± 0.06 | 14.10 ± 0.13 | 14.33 ± 0.16 | 14.38 ± 0.10 |
| Cortical thickness (μm) | 216 ± 4 | 213 ± 4 | 234 ± 8* | 245 ± 9** |
| Periosteal circumference (μm) | 6.92 ± 0.09 | 6.87 ± 0.06 | 8.08 ± 0.36** | 7.90 ± 0.27** |
| Marrow area (mm2) | 0.95 ± 0.03 | 0.94 ± 0.01 | 1.22 ± 0.09** | 1.13 ± 0.06* |
| Cortical area (mm2) | 0.84 ± 0.02 | 0.82 ± 0.02 | 1.05 ± 0.08* | 1.07 ± 0.06** |
| Mean polar moment of inertia (mm4) | 0.38 ± 0.01 | 0.36 ± 0.02 | 0.64 ± 0.09** | 0.61 ± 0.07* |