Figure 7.

A, native MS of intact oligomeric assemblies of WT, S59D (phosphomimic), and D109A (isoAsp-mimic) αB, which are not directly assignable due to overlap of a multitude of charge states and stoichiometries. B, native MS with CID of the ions from A. Shading above shows regions where oligomers (n-mers) have lost one (n-1) or two (n-2) subunits. Detailed view of the region highlighted in green shows that CID resolves the charge states of the oligomers. C, reconstructed oligomeric distributions. Data were charge deconvolved and then corrected to account for stripped subunits.