Figure 5.
TDM blocked the anti-mycobacterial activities of AK15 (A) and its isomer AK15-6 (B). Peptides (750 μg/ml), TDM (10, 100, and 1,000 μg/ml), and peptide (750 μg/ml) mixed with 10, 100, or 1000 μg/ml TDM were incubated at 37 °C and shook at 150 rpm for 5 min. Then peptide, TDM, peptide/TDM mixture, or the same volume of vehicle (PBS, DMSO) was incubated with M. tuberculosis H37Rv (∼107 cfu/ml), respectively. The final concentrations of TDM were 1, 10, and 100 μg/ml, and the final concentration of peptides was 75 μg/ml. After incubation at 37 °C for 7 days, the viable mycobacteria were counted. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant. Error bars, S.E.