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. 2019 Mar 26;294(19):7755–7768. doi: 10.1074/jbc.RA118.006939

Figure 1.

Figure 1.

High glucose promotes the expression of GRP78 at the cell surface. A, MCs were treated with HG for the indicated times, and then csGRP78 was assessed by a biotinylation assay as described under “Experimental procedures.” PDGFR was used as a loading control (Con). B, csGRP78 was assessed by biotinylation after HG or mannitol (Man, 24 h, n = 7). C and D, expression of csGRP78 after HG for 24 h was confirmed by flow cytometry (n = 4). E, markers of ER stress were assessed by immunoblotting after HG treatment for the indicated times (n = 3) CHOP, CCAAT-enhancer-binding protein homologous protein. F, the effects of ER stress inhibitors 4-PBA (2.5 μm) or salubrinal (Sal, 30 μm) on HG-induced csGRP78 expression were assessed by biotinylation (n = 5). G, the effect of MTJ-1 down-regulation using siRNA on HG-induced csGRP78 expression was assessed by biotinylation (n = 3). MTJ-1 down-regulation was assessed by immunoblotting of whole-cell lysate. *, p < 0.05 HG versus control; ‡, p < 0.05 versus HG.