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. 2019 Apr 12;20(8):1805. doi: 10.3390/ijms20081805

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Capillary density is decreased and capillary supply area in the right ventricle (RV) is increased by MCT treatment. (A) Exemplar images showing intense fluorescent labelling of capillaries (magenta arrows) and less intense labelling of sarcolemma (white arrows) with FITC-conjugated lectin. (B) Capillary locations were digitized (green circles), and the area of tissue supplied by each capillary was calculated by Voronoi tessellation (black lines). (C) Capillary density was reduced in MCT and less so in MCT+BB compared to CON. (D) There were no differences between groups in the ratio of capillaries to muscle fibers. (E) The capillary supply area was greater in MCT and MCT+BB than CON, but was reduced in MCT+BB compared to MCT. n = 6 hearts per group. * p < 0.05, ** p < 0.01, *** p < 0.001.