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. 2019 Apr 18;20(8):1922. doi: 10.3390/ijms20081922

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Timetable and method for the generation of hESC-MSC_SP from hESC_SP. (B) The osteogenic and chondrogenic differentiation of hESC-MSC_SP after loading in demineralized bone matrix (DBM). (C) Diagram summarizing the advantages of spheroid culture platform over the monolayer culture system. The figure is reproduced from the article by Yan et al. 2018 [32], which is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). BM—bone marrow; H&E—Hematoxylin and eosin stain.

(A) Timetable and method for the generation of hESC-MSC_SP from hESC_SP. (B) The osteogenic and chondrogenic differentiation of hESC-MSC_SP after loading in demineralized bone matrix (DBM). (C) Diagram summarizing the advantages of spheroid culture platform over the monolayer culture system. The figure is reproduced from the article by Yan et al. 2018 [32], which is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). BM—bone marrow; H&E—Hematoxylin and eosin stain.